Project

Part:BBa_K2702000:Design

Designed by: Biel Badia Roigé   Group: iGEM18_Aachen   (2018-10-07)


ROR-B with new DNA binding domain from Estrogen Receptor


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We had to choose the parts of the sequences of ROR-B and ER, that we exchanged, carefully. To do so, we took the information about both receptors from the uniprot-database in consideration.


Source

The sequences are from human receptors that we ordered at IDT. The codon usage is adapted to the yeast S. cerevisiae

References